Invitrogen™ Human TNF alpha ProQuantum Immunoassay Kit

ELISA

Product Code	Brand	Quantity
15950483	Invitrogen™ A35601	96 Tests
17123119	Invitrogen™ A356015	5 x 96 Tests

Description

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Assay Range: 0.0128 to 5000 pg/mL
• Conjugate: FAM™
• Immunoassay Kit Format: Proximity-based Immunoassay
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Protein Quantitation
• Gene ID (Entrez): 7124
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Regulatory Status: RUO
• Storage Requirements: Frozen at -20°C
• Test Time: 30 mins

• Assay Sensitivity: <0.01 pg/mL
• Detection Method: Taqman™ Real-time PCR
• Product Type: ProQuantum
• Target Species: Human
• For Use With (Equipment): qPCR Instruments
• Incubation Time: 1 hr.
• Label or Dye: FAM™
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Target: TNF alpha
• Total Assay Time: 2 hrs