Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Specification

Product Type	Substrate
Detection Method	Chemiluminescence
Form	Liquid
Product Line	SuperSignal™
Substrate	Horseradish Peroxidase
Sensitivity	Mid-femtogram
Signal Duration	24 hr.

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems and quantitation. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, enabling multiple film or camera exposures.

SuperSignal West Dura Extended Duration Substrate characteristics include:

• Sensitivity: mid-femtogram
• Stability: 24-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 24 hours
• Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Product Code	Brand	Quantity	Price	??product.searchResults.label.quantityAvail??	Quantity
10445345 Promotion Details	Thermo Scientific™ 34075	100 mL	£432.0 100mL			Add to basket
10220294 Promotion Details	Thermo Scientific™ 34076	200 mL	£706.0 200mL			Add to basket
10398773	Thermo Scientific™ 37071	20 mL	£103.0 20mL			Add to basket