Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

Specification

Format	Tube
GC-Rich PCR Performance	High
For Use With (Application)	Hot-start PCR
Concentration	2X
Fidelity (vs. Taq)	1X
Hot Start	Built-In Hot Start
Size (Final Product)	5 kb or less

Platinum II Hot-Start Green PCR Master Mix also includes two tracking dyes for direct loading of PCR products on gels.

Features

• Innovative buffer enables universal annealing temperature by isostabilizing primer-template duplex structures
• Engineered Taq DNA polymerase confers fast cycling and resistance to common inhibitors
• Hot-start technology enables superior specificity, sensitivity, and yields and allows for room temperature reaction setup
• Green PCR buffer reduces pipetting errors with direct gel loading

Applications

• Amplification of DNA from complex genomic, viral, and plasmid templates
• Amplification and improved yields of GC-rich targets
• RT-PCR
• Genotyping
• High-throughput PCR

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Hot-Start PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Notes

• For applications that require PCR product analysis by absorbance or fluorescence excitation, colorless format is recommended.

Product Code	Brand	No. of Reactions	Color	Price	??product.searchResults.label.quantityAvail??	Quantity
15929082 Promotion Details	Invitrogen™ 14001011	20 Reactions	Green	£4.87 Each			Add to basket
15815066 Promotion Details	Invitrogen™ 14001012	50 Reactions	Green	£79.75 50 reactions			Add to basket
15933592 Promotion Details	Invitrogen™ 14001013	200 Reactions	Green	£311.0 Each			Add to basket
15939082 Promotion Details	Invitrogen™ 14001014	1000 Reactions	Green	£1410.0 Each			Add to basket
15889844 Promotion Details	Invitrogen™ 14000012	50 reactions	Colorless	£79.75 50 reactions			Add to basket
15809854 Promotion Details	Invitrogen™ 14000014	1000 reactions	Colorless	£1408.0 1000 reactions			Add to basket
15899844 Promotion Details	Invitrogen™ 14000013	200 Reactions	Colorless	£311.0 200 reactions			Add to basket