IBA Lifesciences pASK-IBA E. coli vector

pASK-IBA E. coli expression vectors encoding a Strep-tag™II and chloramphenicol resistance

Specification

Format	Suspension
Promoter	Tet
Storage Buffer	TE buffer
Antibiotic Resistance Bacterial	Chloramphenicol
Expression System	E. coli
Replication Origin	F1 and ColE1 origin
Size Range	approx. 3 kb
Protein Tag	Strep-tag II
Cloning Method	Direct Cloning
Concentration	250 ng/μL

pASK-IBA vectors are classic cloning vectors devised for periplasmic or cytoplasmic E. coli expression of recombinant proteins with the Strep-tag™II fused to the C- or N-terminus. They carry the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI) and the inducible tetracycline promoter/operator for the regulated expression of proteins. Some carry the ompA signal for periplasmic secretion of the recombinant protein.The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During secretion into the periplasmic space the ompA signal sequence will be cleaved off.

Product Code	Brand	Vector	Price	??product.searchResults.label.quantityAvail??	Quantity
12806486	IBA Lifesciences 2-1321-000	pASK-IBA2C	£204.0 5µg			Add to basket
12826486	IBA Lifesciences 2-1323-000	pASK-IBA4C	£204.0 5µg			Add to basket
12836486	IBA Lifesciences 2-1324-000	pASK-IBA5C	£204.0 5µg			Add to basket
12846486	IBA Lifesciences 2-1325-000	pASK-IBA6C	£204.0 5µg			Add to basket
12856486	IBA Lifesciences 2-1326-000	pASK-IBA7C	£204.0 5µg			Add to basket
12816486	IBA Lifesciences 2-1322-000	pASK-IBA3C	£204.0 5µg			Add to basket