Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Specification

Classification	Animal Origin-Free
Cell Type	Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells
Format	6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample Type	Plasmid DNA,Synthetic siRNA,RNAi Plasmids (shRNA, miR)
Transfection Technique	Lipid-based Transfection
For Use With (Application)	Transfection
Green Features
Product Line	Lipofectamine
Product Type	Transfection Reagent
Serum Compatible	Yes

This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Product Code	Brand	Quantity	Price	??product.searchResults.label.quantityAvail??	Quantity
15282465 Promotion Details	Invitrogen™ L3000001	0.1 mL	£96.25 0.10mL			Add to basket
15292465 Promotion Details	Invitrogen™ L3000008	0.75 mL	£586.0 0.75mL			Add to basket
15212475 Promotion Details	Invitrogen™ L3000015	1.5 mL	£1002.0 1.50mL			Add to basket
15222475	Invitrogen™ L3000075	5 x 1.5 mL	£4640.0 7.50mL			Add to basket
15232475	Invitrogen™ L3000150	15 mL	£8430.0 15mL			Add to basket