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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Overview
Code: A0

Additional Details:
Additional Details: CAS Number: 64-17-5 Weight: 0.09800kg



Product Code. 15167956

Quantity Price
1 £ 54.0 / 1mL
In stock, ready to ship: 40
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Description and Specification

Specification

Bed Volume 1 mL
Diameter (English) Inner 0.2 in.
Diameter (Metric) Inner 7 mm
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Material Polypropylene
pH Range 3 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Quantity 1 mL
Type Pre-packed Column

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.

Safety and Handling

[Warning]

[{code=H226, sequence=1, description=Flammable liquid and vapour.}, {code=H412, sequence=2, description=Harmful to aquatic life with long lasting effects.}]

[{code=P210, sequence=51, description=Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.}, {code=P501b, sequence=52, description=Dispose of contents/container in accordance with local/regional/national/international regulations.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : ethanol, nickel}, {code=UNKNOWN ACUTE, sequence=2, description=16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.}]

[{code=GHS02, sequence=1, description=FLAME}]


Signal Word
  • Warning

Hazard Statement
  • H226- Flammable liquid and vapour.
  • H412- Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P210- Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P501b- Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental Information
  • MIXTURE LIST- Contains : ethanol, nickel
  • UNKNOWN ACUTE- 16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.