Cytiva HisTrap™ HP Prepacked Columns

Product Code.	Quantity	Diameter (Metric) Inner	unitSize
10431065	5 x 1 mL	7 mm	5mL
11763209	5 mL	16 mm	5mL
11773209	5 x 5 mL	16 mm	25mL
15167956	1 mL	7 mm	1mL

Product Code. 10431065 Supplier Cytiva Supplier No. 17524701

Description

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

• Prepacked with Ni Sepharose™ High Performance medium
• Negligible nickel ion leakage
• Pressure Max: 3 bar
• Column material: polypropylene
• 1 and 5mL columns
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
• Manual operation with syringe, pump or chromatography system

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: 3 to 12

1mL columns

• Bed Dimensions: 7 x 25mm
• Flow velocity: 4mL/min.

5mL columns

• Bed Dimensions: 16 x 25mm
• Flow velocity: 5mL/min.

Spécification

• Bed Volume: 1 mL
• Diameter (English) Inner: 0.2 in.
• Diameter (Metric) Inner: 7 mm
• Description: HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
• Flow Rate: 4 mL/min. (1 mL), 5 mL/min. (5 mL)
• Material: Polypropylene
• pH Range: 3 to 12
• Support Type: 6% Highly Cross-linked Agarose
• Content And Storage: 4°C to 30°C
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• Quantity: 5 x 1 mL
• Type: Pre-packed Column

Sécurité et traitement

Mention d'avertissement

• Warning

Catégorie de danger

• Flammable liquid Category 3

Mention de danger

• H226-Flammable liquid and vapour.
• EUH208-Contains . May produce an allergic reaction.

Conseils de prudence

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Informations supplémentaires

• MIXTURE LIST-Contains : Ethanol, Nickel