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Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Overview
522.69 GBP valid until 2025-06-21
Use promo code "24112" to get your promotional price.

Code: 50

Additional Details:
Additional Details: Weight: 0.00450kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10410355

Quantity Price
1 £ 784.0 / Pack of 2
In stock, ready to ship: 8
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For Research Use Only. All usage must comply with product instructions.
 

  • Description and Specification
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Description and Specification

Specification

Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye ROX (Pre-mixed)
Product Line TaqMan™
Product Type Universal Master Mix II
Purity or Quality Grade UP (Ultra Pure)
Quantity 2 x 5 mL
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
Concentration 2X
Fidelity (vs. Taq) 2 X
Sample Type DNA (Genomic), cDNA
Volume 10 mL

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Uracil-N-glycosylase (UNG)
• Optimized buffer components

Note: This TaqMan Universal Master Mix II contains uracil-N-glycoslyase (UNG).

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix (Cat. No. 4444963), our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

Order Info: Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice.

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