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Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Overview
2584.53 GBP valid until 2025-08-24
Use promo code "24117" to get your promotional price.

Code: 50

Additional Details:
Additional Details: CAS Number: 7732-18-5 Weight: 0.50000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10289814

Quantity Price
1 £ 2950.0 / 5000 units
Estimated Shipment
28-08-2025
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Concentration 5 U/μL
Content And Storage • AmpliTaq Gold DNA Polymerase (5 U/μL), 5 x 200 μL
• 10X PCR Buffer I (with MgCl2), 20 x 1.5 mL

Store at -15°C to -30°C.
Format Tube
GC-Rich PCR Performance Low
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase With Buffer I
Quantity 5 x 1,000 units
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR
Fidelity (vs. Taq) 1X
Hot Start Built-In Hot Start
No. of Reactions 4000 Reactions
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Starting Material DNA

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases as reactants are not wasted in the formation of unintended products.

Features

  • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
  • Time-released thermal activation improves sensitivity in low copy number amplifications
  • Successful multiplex PCR saves time and reagents
  • Includes GeneAmp 10X PCR Buffer I with 15 mM MgCl2
  • Hot-start technology reduces risk of biological contamination

Notes

  • For better performance AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation.
  • When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

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